phalloidin staining Search Results


alexafluor 670 phalloidin  (Cytoskeleton Inc)
95
Cytoskeleton Inc alexafluor 670 phalloidin
Alexafluor 670 Phalloidin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/bio_rxiv__64898__2026__03__21__713333-237-7-10?v=Cytoskeleton+Inc
Average 95 stars, based on 1 article reviews
alexafluor 670 phalloidin - by Bioz Stars, 2026-08
95/100 stars
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acti stain phalloidin 488 cat phdg1  (Cytoskeleton Inc)
96
Cytoskeleton Inc acti stain phalloidin 488 cat phdg1
Acti Stain Phalloidin 488 Cat Phdg1, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/10__1161_slash_atvbaha__118__310997-45-4-3?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
acti stain phalloidin 488 cat phdg1 - by Bioz Stars, 2026-08
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acti stain 555 phalloidin  (Cytoskeleton Inc)
96
Cytoskeleton Inc acti stain 555 phalloidin
Acti Stain 555 Phalloidin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pm38701641-411-46-50?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
acti stain 555 phalloidin - by Bioz Stars, 2026-08
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carl confocal splitter  (Carl Zeiss)
90
Carl Zeiss carl confocal splitter
Carl Confocal Splitter, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pmc08718639-113-14-12?v=Carl+Zeiss
Average 90 stars, based on 1 article reviews
carl confocal splitter - by Bioz Stars, 2026-08
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phalloidin staining methodology  (Beijing Solarbio Science)
90
Beijing Solarbio Science phalloidin staining methodology
Phalloidin Staining Methodology, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/10__1016_slash_j__jpha__2024__101054-99-27-32?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
phalloidin staining methodology - by Bioz Stars, 2026-08
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phalloidin staining  (VANGL2 LTD)
90
VANGL2 LTD phalloidin staining
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Phalloidin Staining, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pmc05399569-72-6-18?v=VANGL2+LTD
Average 90 stars, based on 1 article reviews
phalloidin staining - by Bioz Stars, 2026-08
90/100 stars
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alexa fluor 488® phalloidin actin staining  (Becton Dickinson)
90
Becton Dickinson alexa fluor 488® phalloidin actin staining
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Alexa Fluor 488® Phalloidin Actin Staining, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/10__1089_slash_ten__tea__2019__0051-38-0-9?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
alexa fluor 488® phalloidin actin staining - by Bioz Stars, 2026-08
90/100 stars
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acti-stain phalloidin 488  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH acti-stain phalloidin 488
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Acti Stain Phalloidin 488, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pm39404642__am4c07860_si_001-150-6-9?v=Biozol+Diagnostica+Vertrieb+GmbH
Average 90 stars, based on 1 article reviews
acti-stain phalloidin 488 - by Bioz Stars, 2026-08
90/100 stars
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phalloidin-stained images  (MetaMorph Inc)
90
MetaMorph Inc phalloidin-stained images
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Phalloidin Stained Images, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pm28701425-289-7-15?v=MetaMorph+Inc
Average 90 stars, based on 1 article reviews
phalloidin-stained images - by Bioz Stars, 2026-08
90/100 stars
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phalloidin for f-actin filament staining  (CH Instruments)
90
CH Instruments phalloidin for f-actin filament staining
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Phalloidin For F Actin Filament Staining, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pm32263829-207-21-39?v=CH+Instruments
Average 90 stars, based on 1 article reviews
phalloidin for f-actin filament staining - by Bioz Stars, 2026-08
90/100 stars
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anti-stain tm488 fluorescent phalloidin  (Beijing Solarbio Science)
90
Beijing Solarbio Science anti-stain tm488 fluorescent phalloidin
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Anti Stain Tm488 Fluorescent Phalloidin, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/pmc11253501-73-6-10?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
anti-stain tm488 fluorescent phalloidin - by Bioz Stars, 2026-08
90/100 stars
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phalloidin stained images  (universal imaging inc)
90
universal imaging inc phalloidin stained images
Looptail mutation disrupts actin cytoskeleton regulation. (A-D) <t>Phalloidin</t> staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).
Phalloidin Stained Images, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phalloidin+staining/10__1074_slash_jbc__m404814200-72-5-16?v=universal+imaging+inc
Average 90 stars, based on 1 article reviews
phalloidin stained images - by Bioz Stars, 2026-08
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Image Search Results


Looptail mutation disrupts actin cytoskeleton regulation. (A-D) Phalloidin staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).

Journal: Disease Models & Mechanisms

Article Title: Heterozygous Vangl2 Looptail mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair

doi: 10.1242/dmm.028175

Figure Lengend Snippet: Looptail mutation disrupts actin cytoskeleton regulation. (A-D) Phalloidin staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice. Student's t -test, P >0.05). (G) p-Cfl levels are significantly reduced in both Vangl2 Lp/+ (70%) and Vangl2 Lp/Lp (57%) lungs compared with WT littermates ( n =6 per genotype; three separate gels were run, with each gel containing one lane of lung lysate from each genotype. Each lane contained pooled lysate from two individual mice; Student's t -test, * P <0.05). (H) Western blot of total β-catenin (92 kDa) in the cell membrane and cytosolic fractions of adult lungs, and β-actin (42 kDa) and Gapdh (37 kDa) loading controls. Quantification of β-catenin levels (I) in the membrane fraction showed 55% reduction in Vangl2 Lp/+ mice compared with WT controls ( n =3 per genotype, with samples run on a single gel, Student's t -test, * P <0.05). (J) No significant difference was found in the cytosolic compartment ( n =3 per genotype, with samples run on a single gel; Student's t -test, P >0.05). (K) Western blot of E-cadherin in the cell membrane fraction of adult lungs, and Gapdh (37 kDa) loading control. (L) Quantification of E-cadherin levels in the membrane fraction shows no significant difference between Vangl2 Lp/+ mice and WT controls ( n =6 per genotype, two separate gels were run, each with lung lysate from three individual mice per genotype; Student's t -test, P >0.05). Scale bars: 25 µm (A,B) and 12.5 µm (C,D).

Article Snippet: Looptail mutation disrupts actin cytoskeleton regulation. (A-D) Phalloidin staining of filamentous actin distribution in (A,C) WT and (B,D) Vangl2 Lp/+ at (A,B) E14.5 and (C,D) E18.5. (E) Western blot of Cfl and p-Cfl (both 19 kDa) and Gapdh (37 kDa) in total protein lysate from E14.5 WT, Vangl2 Lp/+ and Vangl2 Lp/Lp lungs. (F) Quantification of total Cfl in WT, Vangl2 Lp/+ and Vangl2 Lp/Lp littermates, revealed no significant differences (mean±s.e.m., n =6 per genotype; 3 separate gels were run, with each gel containing 1 lane of lung lysate from each genotype.

Techniques: Mutagenesis, Staining, Western Blot, Membrane, Control